Sinnvoll24
Rapid Cycle Real-Time PCR Methods and Applications
Quantification
Langbeschreibung
Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
Hauptbeschreibung
Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybrdidization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!
Autor*in:
Carl Wittwer
Art:
Kartoniert
Sprache :
Englisch
ISBN-13:
9783642623172
Verlag:
Springer Verlag GmbH
Erscheinungsdatum:
23.10.2012
Erscheinungsjahr:
2012
Ausgabe:
1/2004
Maße:
24.20x19.30x0.00 cm
Seiten:
223
Gewicht:
470 g