E.coli

The study of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections encompasses many different disciplines, including clinical microbiology, diagnostics, animal ecology, and food safety, as well as the cellular microbiology of both bacterial pathogenesis and the mechanisms of toxin action. E. coli: Shiga Toxin Methods and Protocols aims to bring together a number of experts from each of these varied fields in order to o- line some of the basic protocols for the diagnosis and study of STEC pat- genesis. We hope that our book will prove a valuable resource for the clinical microbiologist as well as the cellular microbiologist. For the clinical microbiologist, our aim is to detail a number of current protocols for the detection of STEC in patient samples, each of which have their own advantages. Chapter 1 provides an introduction into the medical significance of STEC infections. Chapters 2-7 follow with protocols for the diagnosis and detection of STEC bacteria in patient and animal samples.

Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods. Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity.

The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections: An Overview Mohamed A. Karmali Methods for Detection of STEC in Humans: An Overview James C. Paton and Adrienne W. Paton Serological Methods for the Detection of STEC Infections Martin Bitzan and Helge Karch Detection and Characterization of STEC in Stool Samples Using PCR Adrienne W. Paton and James C. Paton Molecular Typing Methods for STEC Haruo Watanabe, Jun Terajima, Hidemasa Izumiya, and Sunao Iyoda STEC in the Food Chain: Methods for Detection of STEC in Food Samples Michael Bülte STEC as a Veterinary Problem: Diagnostics and Prophylaxis in Animals Lothar H. Wieler and Rolf Bauerfeind Cellular Microbiology of STEC Infections: An Overview Frank Ebel and Dana Philpott Analysis of Pathogenicity Islands of STEC Tobias A. Oelschlaeger, Ulrich Dobrindt, Britta Janke, Barbara Middendorf, Helge Karch, and Jörg Hacker Generation of Isogenic Deletion Mutants of STEC Soudabeh Djafari, Nadja D. Hauf, and Judith F. Tyczka Generation of Monoclonal Antibodies Against Secreted Proteins of STEC Kirsten Niebuhr and Frank Ebel Microscopic Methods to Study STEC: Analysis of the Attaching and Effacing Process Stuart Knutton Detection and Characterization of EHEC-Hemolysin Herbert Schmidt and Roland Benz Shiga Toxin Receptor Glycolipid Binding: Pathology and Utility Clifford A. Lingwood Methods for the Purification of Shiga Toxin 1 Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood Methods for the Identification of Host Receptors for Shiga Toxin Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport Frederic Mallard and Ludger Johannes Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin Jae H. Kim Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways Nicola L. Jones Interaction of Shiga Toxin with Endothelial Cells Martin Bitzan and D. Maroeska W. M. te Loo Shiga Toxin Interactions with the Intestinal Epithelium Cheleste M. Thorpe, Bryan P. Hurley, and David W. K. Acheson Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes Christian Menge Animal Models for STEC-Mediated Disease Angela R. Melton-Celsa and Alison D. O''Brien Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC Florian Gunzer, Isabel Hennig-Pauka, Karl-Heinz Waldmann, and Michael Mengel Bovine Escherichia coli O157: H7 Infection Model Evelyn A. Dean-Nystrom Index