Advanced Techniques in Diagnostic Microbiology

InhaltsangabeTechniques.- Automated Blood Cultures.- Urea Breath Tests for Detection of Helicobacter pylori.- Rapid Antigen Tests.- Advanced Antibody Detection.- Phenotypic Testing of Bacterial Antimicrobial Susceptibility.- Biochemical Profile-Based Microbial Identification Systems.- Rapid Bacterial Characterization and Identification by MALDI-TOF Mass Spectrometry.- Probe-Based Microbial Detection and Identification.- Pulsed-Field Gel Electrophoresis.- In Vitro Nucleic Acid Amplification: An Introduction.- PCR and Its Variations.- Non-Polymerase Chain Reaction Mediated Target Amplification Techniques.- Recent Advances in Probe Amplification Technologies.- Signal Amplification Techniques: bDNA, Hybrid Capture.- Detection and Characterization of Molecular Amplification Products: Agarose Gel Electrophoresis, Southern Blot Hybridization, Restriction Enzyme Digest Analysis, and Enzyme-Linked Immunoassay.- Direct Nucleotide Sequencing for Amplification Product Identification.- Microarray-Based Microbial Identification and Characterization.- Diagnostic Microbiology Using Real-Time PCR Based on FRET Technology.- Amplification Product Inactivation.- Applications.- Bacterial Identification Based on 16S Ribosomal RNA Gene Sequence Analysis.- Molecular Techniques for Blood and Blood Product Screening.- Review of Molecular Techniques for Sexually Transmitted Diseases Diagnosis.- Advances in the Diagnosis of Mycobacterium tuberculosis and Detection of Drug Resistance.- Rapid Screening and Identification of Methicillin-Resistant Staphylococcus aureus.- Bead-Based Flow Cytometric Assays: A Multiplex Assay Platform with Applications in Diagnostic Microbiology.- Molecular Strain Typing Using Repetitive Sequence-Based PCR.- Molecular Differential Diagnoses of Infectious Diseases: Is the Future Now?.- Pathogen Detection in the Genomic Era.

Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several âEurooehotâEuro spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field

Preface.- Automated Blood Cultures.- Urea Breath Tests for Detection of Helicobacter pylori.- Rapid Antigen Tests.- Advanced Antibody Detection.- Phenotypic Testing of Bacterial Antimicrobial Susceptibility.- Biochemical Profile-Based Microbial Identification Systems.- Rapid Bacterial Characterization and Identification by MALDI-TOP Mass Spectrometry.- Probe-Based Microbial Detection and Identification.- Pulsed Field Gel Electrophoresis.- In Vitro Nucleic Acid Amplification: An Introduction.- PCR and It¿s Variations.- Non-Polymerase Chain Reaction Mediated Target Amplification Techniques.- Recent Advances in Probe Amplification Technologies.- Signal Amplification Techniques: bDNA, hybrid capture.- Detection and Characterization of Molecular Amplificaiton Products: Agarose Gel Electrophoresis, Southern Blot Hybridization, Restriction Enzyme Digest Analysis and Enzyme-Linked Immunoassay.- Direct Nucleotide Sequencing for Amplification Product Identification.- Microarray-Based Microbial Identification and Characterization.- Diagnostic Microbiology Using Real-time PCR Based on FRET Technology.- Amplification Product Inactivation.- Bacterial Identification Based on 16S Ribosomal RNA Gene Sequence Analysis.- Molecular Techniques for Blood and Blood Product Screening.- Review on Molecular Techniques for Sexually Transmitted Dieseases Diagnosis.- Advance in the Diagnosis of Mycobacterium tuberculosis and Detection of Drug Resistance.- Rapid Screening and Identification of Methicillin-Resistant Staphylococcus aureus.- Bead-based Flow Cytometric Assays; A Multiplex Assay Platform with Applications in Diagnostic Microbiology.- Molecular Strain Typing Using Repetitive Sequence -Based PCR.- Molecular Differential Diagnoses of Infectious Diseases: Is the Future Now?- Pathogen Detection in the Genomic Era.- Index.