Electrical Manipulation Of Cells

Electrical Manipulation of Cells provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation. For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. Electrical Manipulation of Cells is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.

Discusses both DNA and RNA transfer and includes a chapter on the equipment required to perform this kind of research. Commercially available equipment is described, and circuit diagrams are also provided so that researchers can design their own apparatus.

Preface. Contributors. Electrofusion and electroporation equipment; I.B. Jones, P.T. Lynch, J.B. Power, M.R. Davey. Electrically stimulated membrane breakdown; T. Y. Tsong. Cell Electrorotation; G. Fuhr, R. Hagedorn. Dielectrophoresis: Effect of nonuniform electrical fields on cell movement; F.J. Iglesias, C. Santamaria, F.J. Ascensor, A. Dominguez. Electrofusion of animal cells; J.A. Lucy. Cell-tissue electrofusion; R. Heller, M. Jaroszeski. Electrofusion of plant protoplasts for hybrid plant production; M.G.K. Jones. Gene delivery by membrane electroplation; E. Neumann. Clinical applications of electroporation; S.B. Dev, G.A. Hofmann. Electroportation and transgenic plant production; M. Joersbo, J. Brunstedt. Electromagnetic cell stimulation; D.B. Jones, B. McCleod. Electrostimulation of cells by weak electric currents; A. Goldsworthy. Stimulation of plant cell division and organogenesis by short-term, high voltage electrical pulses; M.R. Davey, N.V. Blackhall, K.C. Lowe, J.B. Power. Index.